Contents

Preface

1.Introduction

1.1 β – D – fructofuranosidase

1.1.1 Catalytic mechanism

1.1.2 Soluble β – D – fructofuranosidase

1.1.3 Immobilized β – D – fructofuranosidase

1.2 Fructooligosacharides (FOS)

1.2.1 Occurrence

1.2.2 Chemical structure

1.2.3 Enzyme mechanisms

1.2.4 Physicochemical properties

2. β-D-Fructofuranosidase production

2.1 Material

2.2 Production line

2.2.1 Process discription

2.2.2 Factors effecting fermentation

2.2.2.1 Time

2.2.2.2 pH

2.2.2.3 others factors

3. Fructooligosaccharides production

3.1 Process

3.1.1 Enzyme production

3.1.2 Enzyme extraction

3.1.3 Substrates

3.1.4 Cell immobilization

3.1.5 Enzyme immobilization

3.1.6 Fructooligosaccharides syntheisis

3.1.7 Fructooligosaccharide purification

3.1.8 Concentration

3.1.9 Sterilization

3.2 Equipment diagram

3.2.1 Laboratorial scale

3.2.2 Industrial scale

4. Application

4.1 β-frucofuranosidase

4.2 FOS

4.2.1 Apllication

4.2.2 Market trend

References

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Supervisor: As.Pro. Le Van Viet Man

Students: Ho Chi Minh City University of Technology

Faculty of Chemical Engineering